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Anal bioanal chem 2002

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Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay times. In the present study, the kinetic problem was solved by including cystamine as a "mediator" between the protein sulfhydryl and Ellman's reagent, as previously applied in an enzymatic thiol assay [9]. Due to its small size, amphiphilic nature, and lack of charge, DTDP quickly reacts with poorly accessible protein sulfhydryls, without any catalysis by cystamine. This is a preview of subscription content, access via your institution. Rent this article via DeepDyve.
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Fiber-optic biosensors – an overview

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Traceability of pH measurements by glass electrode Catalogue en ligne

This article reviews progress and developments during the past five years in the field of optical fiber biosensors. Because of the expense and time constraints associated with modern laboratory analysis, there is a growing need for real-time, low-cost technology that can be used industrially, environmentally, and clinically, and to monitor food processing. Miniaturization, integrated systems, and multianalyte determination have become key aspects of sensor development and efforts in this direction will also be discussed, with some pointers to likely directions of future research in the area. The review will provide information about the analytical characteristics and applications of fiber-optic biosensors classified depending on the biorecognition element employed — enzymes, whole cells, antibodies, nucleic acids, and biomimetic polymers. This is a preview of subscription content, access via your institution.
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Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4′-dithiodipyridine

Analytical and Bioanalytical Chemistry is a peer-reviewed scientific journal publishing research articles in the broad field of analytical and bioanalytical chemistry. In the journal was renamed to Analytical and Bioanalytical Chemistry. Analytical and Bioanalytical Chemistry had a impact factor of 3. The editors of the journal are A.
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Nueva busqueda. Enlace de registro. In most cases the glass and reference electrodes are thereby fashioned into a single probe, the so-called 'combination electrode' or simply 'the pH electrode'.
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